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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes2 Marks
Question
A and B are the two different cloning vectors in two different bacterial colonies cultured in chromogenic substrate, Bacterial colonies with cloning vector A were colourless, whereas those with B were bluecoloured. Explain giving reasons the cause of the difference in colour that appeared.
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Answer

  • Bacterial colonies with cloning vector A are colourless, as they are recombinants with the insert.
  • Presence of the insert has resulted in insertional inactivation of the enzyme and hence, do not produce any colour.
  • Bacterial colonies with cloning vector B are non recombinants, i.e. have no insert and produce colour as the enzyme is produced.

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