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STD 12 - 9. biotechnology principlas and process — NEET STD 11 Science — Question

Gujarat BoardEnglish MediumSTD 11 ScienceNEETSTD 12 - 9. biotechnology principlas and process4 Marks
MCQ
Assertion : Insertion of recombinant $DNA$ within the coding sequence of $\beta -$ galactosidase results in colourless colonies.

Reason : Presence of insert results in inactivation of enzyme $\beta -$ galactosidase known as insertional inactivation.

  • ✓
    If both Assertion and Reason are correct and the Reason is a correct explanation of the Assertion.
  • B
    If both Assertion and Reason are correct but Reason is not a correct explanation of the Assertion.
  • C
    If the Assertion is correct but Reason is incorrect.
  • D
    If both the Assertion and Reason are incorrect.
✓

Answer

Correct option: A.
If both Assertion and Reason are correct and the Reason is a correct explanation of the Assertion.
a
Alternative markers have been developed that can differentiate recombinants from non recombinants based upon their ability to produce colour in presence of a chromogenic substrate. The plasmid in the bacteria, lacking an insert produces blue coloured colonies, while those plasmids with an insert do not produce any colour due to insertional inactivation of the enzyme, $\beta -$ galactosidase.

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