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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Gujarat BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes1 Mark
MCQ
At which temperature a $\text{DNA}$ molecule is denatured by heat in $\text{PCR}:$
  • A
    $ 70^{\circ}-80^{\circ} \mathrm{C} $
  • ✓
    $ 90^{\circ}-95^{\circ} \mathrm{C} $
  • C
    $ 42^{\circ} \mathrm{C} $
  • D
    $ 50^{\circ}-65^{\circ} \mathrm{C} $
✓

Answer

Correct option: B.
$ 90^{\circ}-95^{\circ} \mathrm{C} $
Denaturation$:$ The reaction temperature is increased to $95$ degree centigrade, which melts $($disrupts the hydrogen bond between complementary bases$)$ all $\text{dsDNA}$ into single$-$stranded $\text{DNA (ssDNA)}.$

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