Question
Classify restriction enzymes based on their mode of action.

Answer

Based on their mode of action restriction enzymes are classified into Exonucleases and Endonucleases.

a. Exonucleases are enzymes which remove nucleotides one at a time from the end of a DNA molecule,
e.g. Bal 31 and Exomiclease III.

b. Endonucleases are enzymes which break the internal phosphodiester bonds within a DNA molecule,
e.g. Hind II, EcoRI, Pvul, BamHI and TaqI.

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