Question
Compare Transmission Electron Microscope with Scanning Electron Microscope.
Features | Transmission Electron Microscope | Scanning Electron Microscope |
Source of illumination for Image Formation | Electrons | Electrons |
Types of cells visualized | Thin sections of the specimen are obtained. The electron beam pass through the sections and form an image with high magnification and high resolution | The specimen is coated with gold and the electrons are reflected back and give the details of surface topography of the specimen |
Image | 2-D | 3-D |
Nature of Lenses | One electrostatic lens with few electromagnetic lenses | One electrostatic lens with few electromagnetic lenses |
Medium | Vacuum | Vacuum |
Specimen mounting | Mounted on coated or uncoated copper grids | Mounted on aluminium stubs and are coated in gold |
Focusing and Magnification Adjustments | Electrical, using deflection coil | Electrical, using deflection coil |
Means for obtaining specimen Contrast | Electron scattering | Electron scattering |
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