MCQ
Consider the following statements.
i. In microinjection method, foreign DNA is directly injected into the nucleus of animal cell or plant cell by using microneedles or micropipettes.
ii. Microinjection method is used in oocytes, eggs, and embryo.
iii. Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride.
iv. In chemical mediated gene transfer method, certain chemicals such as $Ca ^2+$ help foreign DNA to enter the host cell.
Which of the statements given above are correct?
  • A
    (i), (ii), (iii), and (iv)
  • (i), (ii), and (iv)
  • C
    (ii), (iii), and (iv)
  • D
    (i) and (ii)

Answer

Correct option: B.
(i), (ii), and (iv)
(B)(i), (ii), and (iv)
Explanation:
In microinjection, DNA is directly injected into plant protoplasts or cells. This method of gene transfer is used to introduce DNA into large cells such as oocytes, eggs, and the cells of early embryo. Electroporation involves a pulse of high voltage applied to
protoplasts/cells/tissues to make transient (temporary) pores in the plasma membrane which facilitates the uptake of foreign DNA.

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