- AHe repeated each experiment several times.
- BTraits chosen by him had genes far apart so that linkage was absent.
- CHe kept record of all experiments.
- DHe used statistical techniques.
Explanation:
Mendel chose seven pairs of contrasting traits, the gene for each of which is present on seven different chromosomes of pea plant. Due to the presence on different chromosomes, Mendel's trait followed independent assortment during meiosis and produced the expected parental and recombinant phenotypes. If genes of these traits would have present together showing linkage, Mendel would not have got the expected results. Repeating the experiment, keeping records and use of statistical analysis were the secondary factors; major being the chosen traits not showing linkage.
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$I.$ Eco. $RI$ restriction endonuclease enzyme is isolated from Escherichia coli $RY13$
$II.$ Its recognition sequence is $5^{\prime}-$ $GAATTC - 3 '$
$3^{\prime}-C T T A A G-5^{\prime}$
$5^{\prime}-G-A-A-T-T-C-3^{\prime}$
$3^{\prime}-C-T-T-A-A-G-5^{\prime}$
$III.$ Its site of cleavage is