MCQ
CsOH gradient
- A14NH4Cl
- B15NH4Cl
- C35SO2
- DCsCl gradient
Explanation:
In the following procedure, centrifugation is done on a CsCl gradient, the DNA separate out at a point when density equals that of the salt solution.
15NH4Cl was used as a heavy isotope of ammonium chloride while, 14NH4Cl was used as the light isotope of ammonium chloride & the type of centrifugation used was density gradient centrifugation in a CsCl medium.
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$I.$ Eco. $RI$ restriction endonuclease enzyme is isolated from Escherichia coli $RY13$
$II.$ Its recognition sequence is $5^{\prime}-$ $GAATTC - 3 '$
$3^{\prime}-C T T A A G-5^{\prime}$
$5^{\prime}-G-A-A-T-T-C-3^{\prime}$
$3^{\prime}-C-T-T-A-A-G-5^{\prime}$
$III.$ Its site of cleavage is
$(a)$ Population density is necessarily measured in numbers
$(b)$ Tiger census is often based on pug marks and fecal pellets
$(c)$ Biomass is not a meaningful measure to know population size.
$(d)$ Size of a population for any species is not a static parameter