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Molecular Basis of Inheritance — BIOLOGY STD 12 Science — Question

Gujarat BoardEnglish MediumSTD 12 ScienceBIOLOGYMolecular Basis of Inheritance1 Mark
MCQ
CsOH gradient
  • A
    ${ }^{14} \mathrm{NH}_4 \mathrm{Cl}$
  • B
    ${ }^{15} \mathrm{NH}_4 \mathrm{Cl}$
  • C
    ${ }^{35} \mathrm{SO}_2$
  • ✓
    CsCl gradient
✓

Answer

Correct option: D.
CsCl gradient
Explanation: (D) CsCl gradient
In the following procedure, centrifugation is done on a CsCl gradient, the DNA separate out at a point when density equals that of the salt solution.
${ }^{15} \mathrm{NH}_4 \mathrm{Cl}$ was used as a heavy isotope of ammonium chloride while, ${ }^{14} \mathrm{NH}_4 \mathrm{Cl}$ was used as the light isotope of ammonium chloride & the type of centrifugation used was density gradient centrifugation in a CsCl medium.

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