Question
Define the following terms in relation to proteins:
- Peptide linkage.
- Denaturation.
Peptide linkage: Peptide linkage is an amide (-CO-NH-) bond formed between –COOH and –NH2 group in protein formation.
Denaturation: When a protein in its native form, is subjected to physical change like change in temperature or chemical change like change in pH, protein loses its biological activity. This is called denaturation of protein.
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| Experiment. No. | Initial [NO] (M) | Initial [Cl2] (M) | Initial rate of disappearance of Cl2 (M/min) |
| 1 | 0.15 | 0.15 | 0.60 |
| 2 | 0.15 | 0.30 | 1.20 |
| 3 | 0.30 | 0.15 | 2.40 |
| 4 | 0.25 | 0.25 | ? |