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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Rajasthan BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes2 Marks
Question
Describe the steps of recombinant DNA Technology.
✓

Answer

→ Recombinant DNA technology involves several steps in specific sequence such as
(1) Isolation of DNA, fragmentation of DNA, isolation of a desired DNA fragment.
(2) ligation of the DNA fragment into a vector and formation of recombinant DNA.
(3) Transferring the recombinant DNA into the host cell.
(4) Culturing the host cells and selection of clones.
(5) Expression of genes in host cell and obtaining the gene product.

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