Download App

Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Gujarat BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes3 Marks
Question
DNA being hydrophilic, cannot pass through the cell membrane of a host cell. Explain how the recombinant DNA gets introduced into the host cellto transform the latter.
✓

Answer

Introduction of rDNA into host cell:
  1. Microinjection: In this method, the recombinant DNA is directly injected into the nucleus of an animal cell.
  2. Gene gun/ Biolistics: In this method, used for plant cells, the cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA.
  3. Heat shock method: In this method, the rDNA is forced into the competent cell by incubating the cell with rDNA on ice followed by placing them briefly at 42°C (heat shock) and then putting them back on Ice.

Need a full question paper?

Generate a complete, print-ready paper with questions like this in minutes — across 16+ boards, with answer keys.

Start Generating Free

Explore more

More "3 Marks Question" from Biotechnology: Principles and Processes→Biotechnology: Principles and Processes — all question types→BIOLOGY STD 12 Science question papers→Gujarat Board STD 12 Science question papers→Gujarat Board question paper generator→

Similar questions

Enlist the probable causes of infertility.
→
The image below shows two germinated seeds X and Y which belong to the same species. Seed X is produced by apomixis whereas seed Y is a product of sexual reproduction.
Image
A. Write the number of embryo(s), embryo sac(s) and ovules in the ovary of seed X.
B. How multiples embryos are formed in citrus fruits?
C. What advantage will plants developed from seed Y have over seed X?
→
Given below are pairs of diseases and causative organisms. Which out of these is not a matching pair and why?
Filariasis: Wucheraria
Ringworm: Ascaris
AIDS: Human Immuno Virus
Malaria: Plasmodium
→
What is GMO? List any five possible advantages of a GMO to a farmer.
→
Explain into how many categories the endangered species have been classified on the basis of threatened species.
→
  1. What do ‘Y and ‘B’ stand for in ‘YAC’ and ‘BAC’ used in Human Genome Project (HGP)? Mention their role in the project.
  2. Write the percentage of the total human genome that codes for proteins and the percentage of discovered genes whose functions are known as observed during HGP.
  3. Expand ‘SNPs’ identified by scientists in HGP.
→
Explain how biomagnification of DDT occurs in an aquatic food chain.
→
Study the pedigree chart given below, showing the inheritance pattern of blood groups in a family and answer the given questions.
  1. Give the possible genotypes of the individuals 1 and 2.
  2. Which antigen will be present on the plasma membrane of RBCs of individuals 5 and 9?
  3. Give the genotypes of the individuals 3 and 4.
→

  1. Write your observations on the variations seen in the Darwin’s finches shown above.
  2. How did Darwin explain the existence of different varieties of finches on Galapagos Islands?
→
A student on a school trip started sneezing and wheezing soon after reaching the hill station for no explained reasons. But, on return to the plains the symptoms disappeared. What is such a response called? How does the body produce it?
→