Question
Do you know any step in the TCA cycle where there is substrate level phosphorylation. Which one?

Answer

In TCA cycle, coenzyme A split off from succinyl CoA and forms succinic acid. In this process one molecule of GTP (guanosine triphosphate) is formed which later gives one molecule of ATP. This reaction is also referred to as substrate level phosphorylation. $\text{Succinyl}\ \text{CoA}+\text{GDP}+\text{H}_3\text{PO}_4+\text{H}_2\text{O}\\\xrightarrow[\text{thiokinase}]{\text{Succinate}}\ \text{Succinic Acid}+\text{GTP}+\text{CoA}\\\text{GTP}+\text{ADP}\rightarrow\text{GDP}+\text{ATP}$

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