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Molecular Basis of Inheritance — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYMolecular Basis of Inheritance1 Mark
MCQ
During Meselson and Stahl's experiments, heavy $\text{DNA}$ was distinguished from normal $\text{DNA}$ by which type of centrifugation?
  • A
    $CsOH$ gradient
  • B
    $^{14}NH_4​Cl$
  • C
    $^{15}NH_4​Cl$
  • ✓
    $CsCl$ gradient
✓

Answer

Correct option: D.
$CsCl$ gradient
In the following procedure, centrifugation is done on a $CsCl$ gradient, the $DNA$ separate out at a point when density equals that of the salt solution.
$^{15}NH_4Cl$ was used as a heavy isotope of ammonium chloride while, $^{14}NH_4Cl$ was used as the light isotope of ammonium chloride & the type of centrifugation used was density gradient centrifugation in a $CsCl$ medium.

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