MCQ
During PCR technique the pairing of primers to ssDNA segment is called:
- ADenaturation
- BAnnealing
- CPolymerisation
- DIsolation
Explanation:
PCR - technique amplify the DNA sample in three steps namely denaturation of target DNA, annealing and polymerization. Denaturation refers to the thermal cycle that separates the DNA strands and forms the ssDNA. Annealing refers to the pairing of primers to the ssDNA and polymerisation is the extension of primer into complete DNA strand complementary to the template strand.
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| $(a)$ Lactobacillus | $(i)$ Cheese |
| $(b)$ Saccharomyces cerevishe | $(ii)$ Curd |
| $(c)$ Aspergillus niger | $(iii)$ Citric Acid |
| $(d)$ Acetobacter acet $\quad$ | $(iv)$ Bread |
| $(v)$ Acetic Acid |
Select the correct option.
$(a)\quad (b)\quad (c)\quad (d)$