Download App

Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Gujarat BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes3 Marks
Question
Enumerate the steps which are involved in recombinant DNA technology.
✓

Answer

  1. Isolation of DNA.
  2. Fragmentation of DNA by restriction endonucleases.
  3. Isolation of desired DNA fragment.
  4. Ligation of DNA fragment into vector.
  5. Transferring recombinant DNA into host.
  6. Culturing host cells at large scale.
  7. Extraction of the desired product.

Need a full question paper?

Generate a complete, print-ready paper with questions like this in minutes — across 16+ boards, with answer keys.

Start Generating Free

Explore more

More "3 Marks Question" from Biotechnology: Principles and Processes→Biotechnology: Principles and Processes — all question types→BIOLOGY STD 12 Science question papers→Gujarat Board STD 12 Science question papers→Gujarat Board question paper generator→

Similar questions

  1. Name the kind of diseases/ disorders that are likely to occur in humans if
    1. Mutation in the gene that codes for an enzyme phenyl alanine hydrolase occurs,
    2. There is an extra copy of chromosome 21,
    3. The karyotype is XXY.
  2. Mention any one symptom of the diseases/ disorders named above.
→
Explain the mode of action of ECORI.
→
During a visit to Vaishno Devi, Rahul came across a young couple staying in the adjacent room in the hotel. He learnt that the couple had been visiting different temples and performing rituals to get a child. Rahul was astonished and explained to them about ART which he had recently studied in their wrong approach and thanked Rahul.
  1. What is ART? What are the various methods included in ART?
  2. What are the limitations for which ART is not commonly accepted?
  3. Identify the values which Rahul showed.
→
Fertilisation is essential for production of seed, but in some angiosperms, seeds develop without fertilisation.
  1. Give an example of an angiosperm that produces seeds without fertilisation Name the process.
  2. Explain the two ways by which seeds develop without fertilisation.
→
Mention the role of the following in E.coli cloning vector pBR 322.
  1. Selectable marker.
  2. ori.
  3. rop.
→
State Hardy-Weinberg Principle and give the algebraic equation for the distribution of genotypes.
→
Where does fertilisation normally take place in a human female?
→
What is the 'wise use concept of Ramsar Convention? Name four types of wetlands included in its mission.
→
In a typical nucleus some regions of chromatin are stained light and others dark. Explain why it is so and what is its significance.
→
Your classmate complains of headache and cough. The doctor confirms that he is suffering from Pneumonia and not common cold, on the basis of certain symptoms. List these symptoms. Mention any two precautions to be followed to prevent the spread of this disease.
→