Enzymes necessary for recombinant DNA technology are:

MCQ

Enzymes necessary for recombinant $\text{DNA}$ technology are:

A
Endonucleases and polymerases
✓
Restriction endonucleases and ligases
C
Peptidases and ligases
D
Restriction endonucleases and topoisomerases

✓

Answer

Correct option: B.

Restriction endonucleases and ligases

Recombinant $\text{DNA}$ technology is the method of joining two or more $\text{DNA}$ molecules to create a hybrid. This technology is made possible by two types of enzymes, restriction endonucleases and ligase. A restriction endonuclease recognizes a specific sequence of $\text{DNA}$ and cuts within, or close to, that sequence. $\text{DNA}$ fragments generated by digestion with a restriction endonuclease can be joined together again by the enzyme ligase.

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