Download App

Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Gujarat BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes2 Marks
Question
Explain briefly:
PCR
✓

Answer

PCR = Polymerase chain reaction (in vitro method) is a molecular biological technique for enzymatically replicating DNA without using a living organism, such as E. coli or yeast.

3 steps in PCR are-

  1. Denaturation of desired double strand DNA-to ssDNA
  2. Annealing of primer to ssDNA (single standard).
  3. Extension of primer by Taq DNA polymerase isolated form Thermits aquaticus.

Uses- Amplification of desired gene/ gene cloning.

Advantage- More output, greater efficiency, less error prone, less human interference and cyclic and automated.

Need a full question paper?

Generate a complete, print-ready paper with questions like this in minutes — across 16+ boards, with answer keys.

Start Generating Free

Explore more

More "2 Marks Questions" from Biotechnology: Principles and Processes→Biotechnology: Principles and Processes — all question types→BIOLOGY STD 12 Science question papers→Gujarat Board STD 12 Science question papers→Gujarat Board question paper generator→

Similar questions

In Snapdragon, a cross between true-breeding red flowered (RR) plants and true breeding white flowered (rr) plant showed a progeny of plants with all pink flowers.
  1. The appearance of pink flowers is not known as blending. Why?
  2. What is this phenomenon known as?
→
If you squeeze a seed of orange you might observe many embryos of different sizes? How is it possible? Explain.
→
What measures will you take to prevent the spread of water borne diseases?
→
Select two pairs from the following which exhibit divergent evolution. Give reasons for your answer.
  1. Forelimbs of cheetah and mammals.
  2. Flippers of dolphins and penguins.
  3. Wings of butterflies and birds.
  4. Forelimbs of whales and mammals.
→
Why is nascent oxygen supported to be toxic to aerobic life forms?
→
Why is a person with cuts and bruises following an accident administered tetanus antitoxin? Give reasons.

OR

Why does a doctor administer tetanus antitoxoid and not a tetanus vaccine to a child injured in a roadside accident with a bleeding wound? Explain.

→
pairs of contrasting characters of:
  1. Position of flowers.
  2. Colours of pod.
  3. Shape of pod.
  4. Colour of seed, that were selected in pea plant by Mendel.
→
  1. Name the source plant of heroin drug. How is it obtained from the plant?
  2. Write the effects of heroin on the human body.
→
Write the cellular contents carried by the pollen tube. How does the pollen tube gain its entry into the embryo sac?
→
n the given figure of a typical dicot embryo, label the parts (1), (2) and (3). State the function of each of the labelled part.

→