Question
Explain briefly:
PCR
PCR
✓
Answer
PCR = Polymerase chain reaction (in vitro method) is a molecular biological technique for enzymatically replicating DNA without using a living organism, such as E. coli or yeast.
3 steps in PCR are-
Advantage- More output, greater efficiency, less error prone, less human interference and cyclic and automated.
3 steps in PCR are-
- Denaturation of desired double strand DNA-to ssDNA
- Annealing of primer to ssDNA (single standard).
- Extension of primer by Taq DNA polymerase isolated form Thermits aquaticus.
Advantage- More output, greater efficiency, less error prone, less human interference and cyclic and automated.
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