Question
Explain how suspension culture is prepared from callus.
✓
Answer
Explanation:
For the preparation of suspension culture, callus is transferred to a liquid nutrient medium and is agitated. Cells of callus are separated due to agitation. Constant agitation at 100- 250 rpm serves the purpose of aeration, mixing and prevention of aggregation. This suspension culture is sub-cultured every week. Suspension cultures grow here more or less need to be sub-cultured rapidly than the callus culture.
For the preparation of suspension culture, callus is transferred to a liquid nutrient medium and is agitated. Cells of callus are separated due to agitation. Constant agitation at 100- 250 rpm serves the purpose of aeration, mixing and prevention of aggregation. This suspension culture is sub-cultured every week. Suspension cultures grow here more or less need to be sub-cultured rapidly than the callus culture.
Need a full question paper?
Generate a complete, print-ready paper with questions like this in minutes — across 16+ boards, with answer keys.
Explore more
Similar questions
Name a free-living and a symbiotic bacterium that serve as bio-fertiliser. Why are they so called?
Enumerate the symptoms of common cold. Name the category of the organism causing it.
- List two advantages of keeping beehives in a crop field during flowering season.
- Name one annual and one perennial crop species favourable to beeswax collection.
How does Darwin’s theory of Natural Selection explain the appearance of new forms of life on earth?
How many microsporangia are present in a typical anther of an angiosperm?
Distinguish between Homo erectus and Neanderthal man
Name the interaction in the following:
Mycorrhizae living on the roots of higher plants.
Mycorrhizae living on the roots of higher plants.
How are primrry consumers dlfferent from primary carnivores?
Describe the mutual relationship between the fig tree and wasp and comment on the phenomenon that operates in their relationship.
a. Identify A. Which enzyme joined them?
b. Identify B. What is its function ?
