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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes3 Marks
Question
Explain the basis on which the gel electrophoresis technique works. Write any two ways the products obtained through this technique can be utilised.
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Answer

  • In gel electrophoresis, the molecules resolve according to their molecular size by the sieving effect provided by the gel.
  • DNA being negatively charged, moves towards the anode in the electric field.
  • Use of DNA isolated:
  1. The DNA fragments may be used to construct recombinant DNA by joining them with cloning vectors.
  2. The desired DNA fragments may be amplified, i.e. making multiple copies, by polymerase chain reaction (PCR).

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