Question
Explain the three steps carried out in the formation of recombinant DNA using the enzyme EcoRI.
✓
Answer
Eco R1 cuts vector DNA, foreign DNA/ gene of interest, at pallindromic site,
3'CTTAAG 5'
5'GAATTC 3'
(between bases G & A only), sticky end (over hanging stretch of bases) formed at each strand, Joining of sticky ends from DNA fragments by enzyme DNA Ligase, Recombinant DNA(rDNA) is formed.
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