Biotechnology and its Applications — BIOLOGY STD 12 Science — Question

Mass spectrometry-based peptide amide deuterium exchange techniques, have helped to study protein structure and function and are unparalled in their ability to probe sub-molecular protein dynamics. However, this methodology has remained labour-intensive, time consuming and also has comprehensiveness (the extent to which target protein sequence is covered with-measurable peptide fragments) and resolution (the degree to which exchange measurement can be described to particular amides). With Deuterium Exchange Mass Spectrometry (DXMS), complete sequence coverage and single - amide (amino acid) resolution are now rapidly accomplished. DXMS is designed to work well with large proteins and when only small amounts of material are available for study. Studies can be done on receptor - ligand pair as they exist within or on a living cell without prior purification, allowing effective insitu study of integral membrane protein receptors. The major problem is that, it can lead to cancer-related diseases.