Question
For desirable cloning, vector must have origin of replication- discuss your answer.
✓
Answer
→ Origin of replication is a specific genome sequence, from where replication is originated.
→ When any gene or segment of DNA linked with this Ori, unable the replication of that gene in the host cell.
→ This sequence is responsible to maintain copies of linked DNA.
→ If we want high number of clones then we have to select the vector in a such way which favours the gene to produce high number of copies.
→ When any gene or segment of DNA linked with this Ori, unable the replication of that gene in the host cell.
→ This sequence is responsible to maintain copies of linked DNA.
→ If we want high number of clones then we have to select the vector in a such way which favours the gene to produce high number of copies.
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