Question
Gene expression can be controlled with the help of RNA. Explain the method with an example.

Answer

Gene expression can be controlled with the help of RNA interference (RNAi). RNAi takes place in all eukaryotic organisms and is part of the cellular defense. Under this method, a complementary dsRNA binds to and prevents translation of mRNA (silencing) and thus silences a specific mRNA. Thus gene expression which could have been carried out by the mRNA does not happen.
This method has been used to encounter a nematode in plants. Agrobacterium is used as vector to introduce nematode specific gene into the host plant. The introduction of DNA results in production of sense and anti-sense RNA in the host cells. These two RNAs form dsRNA and silence the specific mRNA.

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