Question
- How are recombinant vectors created?
- For creating one recombinant vector only one type of restriction endonuclease is required. Give reason.
✓
Answer
- The vector DNA is cut at a particular restriction site using a restriction enzyme (to cut the desired DNA segment). The alien DNA is then linked with the plasmid DNA using an enzyme called ligase to form the recombinant vector.
- A restriction enzyme recognizes and cuts the DNA at a particular sequence, called recognition site. If more than one restriction enzymes are present, they will generate several segments and will complicate gene cloning.
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