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Enhancement of Food Production — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYEnhancement of Food Production3 Marks
Question
How aseptic conditions are maintained in tissue culture?
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Answer

  1. Glassware is sterilized by using detergents and hot air oven.
  2. Nutrient medium is autoclaved under constant pressure of $15$ lb/sq inch, continuously for $20$ minutes to sterilize it.
  3. Explant is treated with $20\%$ ethyl alcohol and $0.1\% HgCl_2.$
  4. Sterilization of inoculation chamber $($ Laminar air flow $)$ is done using $UV$ ray tube for $1$ hour before actual inoculation of explant on the sterilized nutrient medium.

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