Question
How did the process of RNA interference help to control the nematode from infecting the roots of tobacco plants?
Or
How has RNAi technique helped to prevent the infestation of roots in tobacco plants by a nematode Meloidogyne incognita?✓
Answer
RNA interference is a gene-silencing process that blocks the expression of genes in the parasite when it enters the host's body. RNAi is a method adopted to prevent infestation of roots of tobacco plants by a nematode meloidegyne incognitia. In RNAi, a complementary RNA binds to mRNA to from a ds RNA that cannot translate and hence, its expression is blocked (Silencing). In this process, nematode-specific genes (DNA) are introduced in the host plant. This introduced DNA forms both sense and anti-sense RNA. These two stands, being complementary to each other, bend and form ds RNA, leading to RNA interface. mRNA of nematode is thus silenced and the parasite cannot survive in the transgenic host. Thus, through the above method, tobacco plants can be protected from nematode attack.
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