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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes3 Marks
Question
How do antibiotic-resistance genes function as selectable markers? Explain with the help of E.coli cloning vector pBR 322.
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Answer

  • If a foreign DNA is ligated at the BamH I site of tetracyclin-resistance gene in the pBR 322, the recombinant plasmid will lose the tetracycline-resistance.
  • The transformant can be selected out from the nontransformants by culturing on a medium containing ampicillin.
  • The transformants are transferred to a culture medium containing tetracyclin; there they fail to grow but non-transformants grow in tetracyclincontaining medium as well as ampicillin-containing medium.
  • Here, one antibiotic gene facilitates selection of transformants, while the other antibiotic gene gets inactivated by the insertion of alien DNA and facilitates selecting the recombinants.

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