Question
- Identify the selectable markers in the diagram of E. coli vector shown above.
- How is the coding sequence of a-galactosidase considered a better marker than the ones identified by you in the diagram? Explain.
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Answer
- a = ampicillin, b = EcoR-I, c = Hind-III, d = tetracycline.
- Insertion of alien gene into coding sequence of a-galactosidase, results into inactivation of the enzyme// (insertion inactivation), these colonies do not produce any colour (in the presence of chromogenic substrate) hence are identified as recombinant colonies.
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