MCQ
If a recombinant $DNA$ bearing gene for ampicillin resistance is transferred into $E.$ coli cells and the host cells are spread on agar plates containing ampicillin, then
  • A
    Both transformed and untransformed recipient cells will die
  • B
    Both transformed and untransformed recipient cells will grow
  • Transformed recipient cells will grow and untransformed recipient cells will die
  • D
    Transformed recipient cells will die and untransformed recipient cells will grow

Answer

Correct option: C.
Transformed recipient cells will grow and untransformed recipient cells will die
c
Transformation is a procedure through which a piece of DNA is introduced in a host bacterium. DNA bearing ampicillin resistance gene (transformed recipient cells) when introduced into the plate containing ampicillin will grow while DNA not bearing ampicillin resistance gene (untransformed recipient cells) will die. The genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for $E.$ coli The normal $E.$ coli does not carry resistance against any of these antibiotics.

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