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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes3 Marks
Question
List in proper sequence the processes involved in recombinant DNA (rDNA) technology.
✓

Answer

The steps are:
  1. Isolation of DNA.
  2. Fragmentation of DNA by restriction endonucleases.
  3. Isolation of a desired DNA fragment.
  4. Amplification of the desired DNA fragment.
  5. Ligation of the DNA fragment into a vector.
  6. Transferring the recombinant DNA into the host.
  7. Culturing the host cells and extraction of the desired gene product on a large scale.
  8. Downstream processing, i.e. separation and purification.

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