Microbes in Human Welfare — BIOLOGY STD 12 Science — Question

Explain pBR322 with its cloning sites, enzymes with well labeled diagram.###Explain: cloning sites. (Diagram is not necessary)

Bacteria cannot be seen with the naked eyes, but these can be seen with the help of a microscope. If you have to carry a sample from your home to your biology laboratory to demonstrate the presence of microbes with the help of a microscope, which sample would you carry and why?

Read the following and answer any four questions from S(i) to S(v) given below:

Rajat is a student of biotechnology. His professor tells him that for transformation with recombinant DNA the bacterial cells must be made capable of taking up DNA as DNA do not pass through membrane. While doing experiment in the lab, Rajat noticed that bacterial cells were not taking up the foreign DNA even after treating it with sodium ion. He asked his professor, the reason behind this. His professor explained that he should check the valency and charge of the ion that he is using for the treatment.

1. It is difficult for DNA to pass through the membrane as

1. It is a hydrophilic molecule.
2. It is a hydrophobic molecule.
3. It is a circular molecule.
4. It changes its shape when it comes in contact with host cell.

2. What type of ions are used for DNA mediated gene transfers?

1. Divalent anions.
2. Divalent cations.
3. Monovalent cations.
4. Monovalent anions.

3. rDNA stands for,

1. reduced DNA
2. red DNA
3. recombinant DNA
4. related DNA.

4. Which of the following statements with regard to DNA is correct?

1. DNA is a positively charged molecule having two polynucleotide chains.
2. Nitrogen bases of two polynucleotide chain form complementary pairs, i.e., A opposite G and T opposite C.
3. Backbone of DNA chain is built up of alternate deoxyribose sugar and phosphate group.
4. Both (a) and (c)

5. Assertion: Competent host is essential for transformation with rDNA.

Reason: Transfer of DNA in a prokaryotic cell is called transfection.

1. Both assertion and reason are true and reason is the correct explanation of assertion.
2. Both assertion and reason are true but reason is not the correct explanation of assertion.
3. Assertion is true but reason is false.
4. Both assertion and reason are false.

Read the following and answer any four questions from (i) to (v) given below:
A 23 year old Sahil has been diagnosed with an infection of reproductive tract caused by bacteria. He complains about burning sensation during urination, pus-containing discharge and pain around genitalia. This infection has incubation period of 2-5 days but can be cured.

1. From which disease is Sahil suffering?

1. Chlamydiasis.
2. Herpes.
3. Gonorrhoea.
4. Syphilis.

2. Which among the following reproductive tract infections is transmitted by bacteria?

1. Trichomoniasis.
2. Chancroid.
3. Genital warts.
4. Both (a) and (b).

3. Which mode of treatment would be useful for Sahil?

1. Cryosurgery.
2. Use of Podophyllum preparation.
3. Use of antibiotic ampicillin.
4. It cannot be treated.

4. What technique was used to diagnose Sahil's disease?

1. Gram staining of discharge and culture.
2. ELISA Test.
3. Antibody detection.
4. Antigen test.

5. Find the odd one out.

1. Treponema pallidum.
2. Neisseria gonorrhoeae.
3. Haernophilus ducreyi.
4. Trichomonas vaginalis.

Explain packaging of DNA Helix###Explain the formation of nucleosome in Eukaryotic cell

What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.

Read the following and answer any four questions from (i) to (v) given below:

During copulation, semen is released by the penis into the vagina. 'The motile sperms swim rapidly, fuse with ovum in the ampullary region, resulting in fertilisation. Haploid nucleus of sperm fuse with that of ovum to form diploid zygote.

1. In female genital tract, sperms are made capable of fertilising the egg. This phenomenon of sperm activation is called:

1. Amphimixis.
2. Cortical reaction.
3. Capacitation.
4. Acrosomal reaction.

2. Select the correct sequence of various physical and chemical events that take place during fertilisation.

16. Fusion of cortical granules with plasma membrane of secondary oocyte.
17. Formation of fertilisation cone to receive sperm.
18. Release of sperm lysin from acrosome.
19. Mixing up of chromosomes of a sperm and an ovum.

1. R → Q → P → S
2. Q → S → R → P
3. Q → R → S → P
4. R → P → Q → S

3. Assertion: Only one sperm can fertilise an ovum.

Reason: The secretion of acrosome help the sperm to enter into cytoplasm of ovum through zona pellucida and plasma membrane.

1. Both assertion and reason are true, and reason is the correct explanation of assertion.
2. Both assertion and reason are true, but reason is not the correct explanation of assertion.
3. Assertion is true, but reason is false.
4. Both assertion and reason are false

4. What is the significance of fertilisation?

1. It restores haploid number of chromosomes.
2. It produces offspring genetically identical to parents.
3. It initiates cleavage.
4. Both (b) and (c).

5. Site of fertilisation in humans is:

1. Endometrium of uterine cavity.
2. Ampullary isthmic junction of oviduct.
3. Cervix of uterus.
4. Infundibulum of fallopian tube.

Explain loss of biodiversity.

Read the following and answer any four questions from (i) to (v) given below:

Intrauterine devices are most widely accepted methods of contraception. These are used by females and are inserted by doctor or nurses in the uterus through vagina. However, these devices are not recommended for those who eventually intend to conceive.

1. How does CuT prevent conception?

1. Cu ions make uterus unsuitable for implantation.
2. Cu ions make cervix hostile to the sperms.
3. Cu ions suppress sperms motility.
4. Cu ions inhibit ovulation.

2. Which of the following IUDs makes uterus unsuitable for implantation?

1. LNG-20.
2. Multiload 375.
3. Cu7.
4. Lippes loop.

3. Identify the correct statement for IUDs.

1. They slowly release synthetic progesterone in the body.
2. They increase phagocytosis of sperms within the uterus.
3. They block entry of sperms through the cervix.
4. Both (b) and (c).

4. Select the correct matched pair.

1. Hormone releasing IUD - LNG-20.
2. Non-medicated IUD - Progestasert.
3. Copper releasing IUD - Lippes loop.
4. None of these.

5. Assertion: IUDs can cause excess menstrual bleeding and pain.

Reason: IUDs can perforate uterus.

1. Both assertion and reason are true, and reason is the correct explanation of assertion.
2. Both assertion and reason are true, but reason is not the correct explanation of assertion.
3. Assertion is true, but reason is false.
4. Both assertion and reason are false.

Read the following text carefully and answer the questions that follow:
Transgenic animals can serve as factories that in some cases, may produce large amounts of proteins more efficiently. Transgenic mice have been engineered to express human antibodies by introducing a large segment of human DNA encoding human immunoglobulin genes. In transgenic large animals such as cows or sheep proteins of pharmaceutical value can be produced in large quantities in milk which is later purified. Transgenes can be used to alter many phenotypic properties including growth rate, fat composition, milk production, hair texture, etc.
i. In transgenic animals, i.e. cow and sheep proteins of pharmaceutical value are produced in large quantities in which gland. (1)
ii. Why is mouse the most preferred animal for studies on gene transfer? (1)
iii. Why does the production of transgenic animals take place? (2)
OR
Assertion (A): Transgenic mice have been engineered to express human antibodies. (2)
Reason (R): Large segments of human DNA encoding human immunoglobulin have been transferred to mice.
a. Both A and R are true and R is the correct explanation of A.
b. Both A and R are true but R is not the correct explanation of A.
c. A is true but R is false.
d. A is false but R is true.