Question
Name the different types of Chromatography and explain the principles underlying them.

Answer

Depending on the nature of the stationary phase i.e., whether it is a solid or a liquid, chromatography is classified into adsorption chromatography and partition chromatography.
i. Adsorption chromatography: This technique is based on the principle of differential adsorption. Different solutes are adsorbed on an adsorbent to different extent.

Adsorption chromatography is further classified into two types:

  1. Column chromatography
  2. Thin-layer chromatography

ii. Partition chromatography: This technique is based on continuous differential partitioning of components of a mixture between stationary and mobile phases. For example, paper chromatography

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