Question
Name the specific enzyme responsible for nucleotide polymerisation in DNA replication. Write two characteristic features of this enzyme. Name the region on E.coli DNA, where this enzyme can initiate replication.
✓
Answer
- DNA-dependent DNA polymerase is the enzyme.
- Two characteristic features of this enzyme include:
- It catalyses the polymerisation of nucleotides in 5' → 3' direction only.
- It polymerises approximately 2000bp per second, with high degree of accuracy.
- Origin of replication is the region on E.coli DNA, where this enzyme can initiate replication.
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