Question
Packaging in Prokaryotes
✓
Answer
- Size of cell in E. coli size is 2-3µ.
- The nucleoid is small, circular, highly folded, naked DNA (1100 µm long in perimeter and contains about 4.6 million base pairs).
- When the negatively charged DNA becomes circular, the size reduces to 350 µm in diameter.
- Folding/looping (40-50 domains (loops) further reduce it to 30 µm in diameter.
- RNA connectors assist in loop formation.
- Further coiling and super coiling of each domain reduces the size to 2 µm in diameter.
- This coiling (packaging) is assisted by positively charged HU (Histone like DNA binding proteins) proteins and enzymes like DNA gyrase and DNA topoisomerase-I, which maintain supercoiled state.
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