- ANicking by restriction endonucleases
- BPolymerase chain reaction
- CReverse genetics
- DSplicing
Explanation:
In 1985 Norm Arnheim, also a member of the development team concluded his sabbatical at Cetus and assumed an academic position at USC. He began to investigate the use of PCR to amplify samples containing just a single copy of the target sequence. By 1989 his lab developed multiplex-PCR on single sperm to directly analyze the products of meiotic recombination.
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$I.$ It is phenomenon in which more recombinants are produced in $F_{2}$-generation
$II.$ More parental combination are produced in $F_{2}$-generation
$III.$ Genotype which are present in $F_{1}$ hybrid. Reappear in high frequency in $F_{2}$-generation
$IV.$ It is a phenomenon in which two chromosome are linked