MCQ
'Restriction' in Restriction enzyme refers to:
- ACleaving of phosphodiester bond in DNA by the enzyme.
- BCutting of DNA at specific position only.
- CPrevention of the multiplication of bacteriophage in bacteria.
- DAll of the above.
Restriction enzymes belong to a class of enzyme called nuclease. Restriction enzymes prevent the multiplication of bacteriophage in bacteria. They are used for cutting of DNA at specific position which becomes possible by cleaving of phosphodiester bond in DNA by the enzyme. Option ‘c’ is the correct answer.
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$R$ : Deoxyribonuclease will digest $DNA$ while $RNA$ will be intact.
| List $-I$ | List $-II$ |
| $a$ In lac operon $i$ gene codes for | $i$ transacetylase |
| $b$ In lac operon $z$ gene codes for | $ii$ permease |
| $c$ In lac operon $y$ gene codes for | $iii$ $\beta$ - galactosidase |
| $d$ In lac operon $a$ gene codes for | $iv$ Repressor |
Choose the correct answer from the options given below :
Reason : Interferons stimulate inflammation at the site of injury.
| Column - $I$ | Column - $II$ |
| $(a)$ Bioreactor | $(i)$ Separation of DNA fragments |
| $(b)$ Electrophoresis | $(ii)$ Production of large quantities of products |
| $(c)$ $PCR$ | $(iii)$ Detection of pathogen, based on antigen-antibody reaction |
| $(d)$ $ELISA$ | $(iv)$ Amplification of nucleic acids |
Select the correct option from following: