MCQ
'Restriction' in Restriction enzyme refers to:
  • A
    Cleaving of phosphodiester bond in DNA by the enzyme.
  • B
    Cutting of DNA at specific position only.
  • C
    Prevention of the multiplication of bacteriophage in bacteria.
  • D
    All of the above.

Answer

  1. Prevention of the multiplication of bacteriophage in bacteria.

Restriction enzymes belong to a class of enzyme called nuclease. Restriction enzymes prevent the multiplication of bacteriophage in bacteria. They are used for cutting of DNA at specific position which becomes possible by cleaving of phosphodiester bond in DNA by the enzyme. Option ‘c’ is the correct answer.

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