Question
The concentration of a substrate is allowed to increase continuously. Explain the effect of this rise on the rate of enzymatic reaction.
✓
Answer
- With the increase in substrate concentration, the velocity of enzyme reaction increases at first.
- But it reaches a maximum velocity $\left(V_{\max }\right)$ at a particular concentration of the substrate.
- Beyond this, there is no further increase in the velocity of the reaction, as all the active sites of the enzyme are saturated; the number of active sites on the enzyme is fewer than the number of substrate molecules.
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