Question
The method developed by Eli Lilly using R-DNA technology has received great support from diabetic patients. Explain, how?
✓
Answer
Eli Lilly prepared two DNA sequences corresponding to the A and B chains of human insulin and introduced them into a plasmid of E. coli for the production of insulin chains A and B. Chains A and B were produced separately, extracted and converted into human insulin. Combined by forming a disulfibond to make insulin. Insulin is synthesized in the human pancreas as a pro-hormone containing a C peptide, which is cleaved into the mature hormone. Insulin synthesized by recombinant DNA technology did not contain the C peptide and was prepared in the mature form.
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