MCQ
To isolate protoplast, one needs.
- AChitinase.
- BCellulase.
- CPectinase.
- ✓Both pectinase and cellulase.
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$I.$ Extraction of the desired gene product
$II.$ Amplification of the gene of interest
$III.$ Isolation of a desired $DNA$ fragment
$IV.$ Ligation of the $DNA$ fragment into a vector
$V.$ Insertion of recombinant $DNA$ into the host
Correct order is