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Biotechnology: Principles and Processes — BIOLOGY STD 12 Science — Question

Maharashtra BoardEnglish MediumSTD 12 ScienceBIOLOGYBiotechnology: Principles and Processes1 Mark
MCQ
Two statements are given $-$ one labelled Assertion and the other labelled Reason. Select the correct answer to these questions from the codes $(a), (b), (c)$ and $(d)$ as given below.
Assertion: The insertion of $\text{DNA}$ fragment into $\text{PBR} \ 322$ plasmid using enzyme Past $I$ or Pvu $I$ make ampicillin resistant gene non functional.
Reason: Bacterial cells containing recombinant $\text{PBR}\ 322$ is unable to grow in the presence of ampicillin.
  • A
    Both assertion and reason are true and reason is the correct explanation of assertion.
  • ✓
    Both assertion and reason are true but reason is not the correct explanation of assertion.
  • C
    Assertion is true but reason is false.
  • D
    Both assertion and reason are false.
✓

Answer

Correct option: B.
Both assertion and reason are true but reason is not the correct explanation of assertion.
Plasmid $\text{PBR} \ 322$ has a variety of unique restriction sites for restriction endonucleases.
Two unique sites, Pst $I$ and Pvu /are located within the amp' gene and Bamrll, Sal $I$ etc. are within $\text{tet}^1$ gene.
The presence of restriction sites within the marker tef and amp' permits an easy selection for cell transformed with the recombinant $\text{PBR} \ 322$. Insertion of the $\text{DNA}$ fragment into the plasmid using enzyme Pst $I$ and Pvu $I$ places the $\text{DNA}$ insert within the gene amp' and make it non functional.

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