Question
What is a recombinant DNA? List its features. How do enzymes restriction endonuclease and DNA Ligase help its formation?
✓
Answer
- The DNA formed by combining DNA molecules from different sources/ genomes, is called a recombinant DNA.
- It shows features of those organisms from where the DNAs are isolated.
- The same restriction endonuclease is used to cut both vector DNA and the source DNA at specific locations: hence, the same kind of sticky ends are formed.
- DNA ligase joins together (end-to-end) the complementary cut counterparts by forming hydrogen bonds.
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