Plant Water Relation — BIOLOGY STD 12 Science — Question

Give definitions : Gene library

Read the following and answer any four questions from (i) to (v) given below:
One approach of gene therapy to treat cystic fibrosis uses viruses to deliver normal alleles of the CFTR gene into epithelial cells of the airways. A team of researchers in the USA developed a new strain of non-pathogenic adeno-associated virus (AAV), AAV2.ST. Genes for the CFTR protein and the enzyme luciferase were inserted into the DNA of the viruses. Luciferase catalyses the production of a green fluorescent protein when luciferin is added. The normal AAV strain and the AAV2.5T strain were added to cultures of epithelial cells from the airways. After adding luciferin, the number of cells that had taken up the viral genes was estimated using the intensity of the green fluorescence which developed. The result are shown in the given graph.

1. What could be the probable reason for inserting a gene for luciferase by researchers into the viral DNA?

1. Infected cells are able to produce luciferase.
2. It is easy to identify the infected cells that have taken up viral DNA under fluorescent lamp.
3. THe non-infected cells were easily identified under fluorescent lamp as they will glow.
4. Both (a) and (b).

2. Select the incorrect statement with respect to the graph given.

1. Both AAV and AAV2.ST can infect epithelial cells.
2. Intensity of green fluorescence increases more in AAV 2.5 T as compared to normal AAV.
3. AA V infect cells more readily than AAV 2.5 T.
4. None of these.

3. The name of first transgenic cow is:

1. cDNA does not contain non-coding regions such as intrans.
2. cDNA can be transcribed and translated directly.
3. There is no need of post-trancriptional modification such as splicing, etc.
4. All of these.

4. There is a decrease in intensity of green fluorescence in cells infected with AAV 2.ST during the last 10 day. This is because:

1. Green fluorescent protein was broken down.
2. Luciferin was used up.
3. Infected cells die.
4. All of these.

5. Which of the following best describe gene therapy?

1. Mutating the sequence of a particular gene.
2. Replacing a healthy gene with a defective gene.
3. Replacing a defective gene with a functional gene.
4. Transferring a healthy gene from one species with heathy gene of other species.

Name the following : Name the term for the transmission of HIV from pregnant mother to foetus.

What is the role of water for growth?

Which are the diseases that can be avoided by eradication of mosquitoes in your area?

Give definitions : Mineralization

In terrestrial ecosystem DFC and GFC are interlinked at certain level. Justify the statement.

Clot formation can be prevented by treatment with_______ in gene therapy:

1. DNAase
2. Recombinant vaccine
3. TPA
4. TGF-B

Which disease is caused by hyper secretion of Glucocorticoids?

Enlist the layers of sporoderm and their composition.