Question
What is rDNA? Explain the technique of cloning rDNA.
✓
Answer
rDNA is the DNA formed by combining DNAs from two different organisms. In bacterial cell, rDNA can be transferred by using a vector where it multiplies into many copies. These copies are preserved as gene library.
Recombinant DNA technology involves the following steps:
Recombinant DNA technology involves the following steps:
- Isolation of DNA.
- Fragmentation of DNA by restriction endonucleases.
- Isolation of a desired DNA fragment.
- Amplification of the gene of interest.
- Ligation of the DNA fragment into a vector.
- Insertion of recombinant DNA into the host.
- Culturing the host cells on a suitable medium at a large scale.
- Extraction of the desired gene product.
- Downstream processing of the products as finished product, ready for marketing.
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