MCQ
What is the first step in the Southern Blot technique
  • A
    Denaturation of $DNA$ on the gel for hybridization with specific probe
  • B
    Production of a group of genetically identical cells
  • C
    Digestion of $DNA$ by restriction enzyme
  • Isolation of $DNA$ from a nucleated cell such as the one from the scene of crime

Answer

Correct option: D.
Isolation of $DNA$ from a nucleated cell such as the one from the scene of crime
d

(d) The first step in southern blot technique is isolation of $DNA$ from a nucleated cell and then these isolated $DNA$ sequences are transferred to a nylon or nitrocellulose sheet placed over the gel.

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