MCQ
What is true about DNA polymerase used in PCR?
- AIt is used to ligate introduced DNA in recipient cells.
- BIt serves as selectable marker.
- CIt is isolated from a virus.
- DIt is active at high temperature.
Explanation:
DNA polymerase enzyme is an essential component for PCR due to its key role in synthesizing new DNA strands. Taq DNA polymerase ( synthesized from the thermophilic bacteria Thermus aquaticus) is the most common enzyme used for PCR amplification. This enzyme is extremely heat resistant with a half-life of 40 minutes at 95°C.
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| Column $- I$ | Column $- II$ |
| $(a)$ Parturition | $(p)$ Attachment of embryo to endometrium |
| $(b)$ Gestation | $(q)$ Release of egg from Graafian follicle |
| $(c)$ Ovulation | $(r)$ Delivery of Foetus from uterus |
| $(d)$ Implantation | $(s)$ Duration between pregnancy and birth |
| $(t)$ Formation of zygote by fusion of egg and sperm |