- ATemplate hypothesis
- BDegeneracy
- CGene flow hypothesis
- DCollinearity hypothesis
Explanation:
The genetic code shows degeneracy which means that more than one triplet can code for same amino acid. For example, phenylalanine is encoded by three triplets; UUU, UUC, UUA.
According to template hypothesis, during transcription, the 3' → 5' strand of DNA serves as template strand to synthesize the RNA molecule which has a sequence of ribonucleotides exactly complementary to that of the template strand. For example, a DNA template strand with 3' TACATGGGTCCG 5' produces mRNA having 5'AUGUACCCAGGC3' sequence. The ribonucleotide sequence of the mRNA is exactly same as that of the coding strand of DNA. The coding strand is 5' → 3' DNA strand which does not serve as the template for RNA synthesis.
Gene flow hypothesis marks the factors responsible for the transfer of genes/alleles from one population to other; migration being the primary agent.
Colinearity hypothesis was given by Beadle and Tatum. It says that genes and proteins show collinear relationship which means that the linear sequence of the nucleotides in the gene determines the linear sequence of amino acids in a protein.
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Assertion $(A):$
Polymerase chain reaction is used in DNA amplification
Reason $(R):$
The ampicillin resistant gene is used as a selectable marker to check transformation
In the light of the above statements, choose the correct answer from the options given below :
$I$. Light $II$. Temp (suitable)
$III$. Moisture $IV$. Oxygen
Select correct option