Human Health and Disease — BIOLOGY STD 12 Science — Question

$I.$ Extraction of the desired gene product

$II.$ Amplification of the gene of interest

$III.$ Isolation of a desired $DNA$ fragment

$IV.$ Ligation of the $DNA$ fragment into a vector

$V.$ Insertion of recombinant $DNA$ into the host

Correct order is

$I$. Lymphocytes are obtained from the patients

$II$. Lymphocytes are transferred to the culture dishes

$III$. Lymphocytes are transfected with the normal $ADA$ genes

$IV$. The transfected cell are returned to the patients

The chronological order should be