Question
Why does the 'insertional inactivation' method to detect recombinant DNA is preferred to 'antibiotic resistance' procedure?
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Answer
The presence of a chromogenic substrate gives blue coloured colonies, in absence of an insert/in non-transformants, presence of an insert (in the enzyme site), results into (insertional inactivation of the β-galactosidase) colonies which do not produce colour.
Antibiotic resistance method requires duplicate plating/cumbersome procedure.
Antibiotic resistance method requires duplicate plating/cumbersome procedure.
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